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Cyclin T1 antibody

Anti-Cyclin T1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22098

Cat. No. GTX22098

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($589)
See all Cyclin T1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:200-1:1000
IP 1:100
ELISA 1:1000-1:5000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Product Note

No reaction was observed against other related cyclins.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to KLH.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin T1 , CCNT , CYCT1 , HIVE1

Cellular Localization

Nucleus

Background

Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.

Database

Research Area

DATA IMAGES

Anti-Cyclin T1 antibody used in IHC (Paraffin sections) (IHC-P). GTX22098

GTX22098 IHC-P Image

IHC-P analysis of human skin tissue using GTX22098 Cyclin T1 antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589