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Cyclin T2 antibody [C2C3], C-term

Anti-Cyclin T2 antibody [C2C3], C-term used in Western Blot (WB). GTX111944
Anti-Cyclin T2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111944
Anti-Cyclin T2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111944
Anti-Cyclin T2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111944

Cat. No. GTX111944

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cyclin T2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human cyclin T2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949897

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin T2 , CYCT2

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Two alternatively spliced transcript variants, which encode distinct isoforms, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclin T2 antibody [C2C3], C-term used in Western Blot (WB). GTX111944

GTX111944 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX111944 diluted at 1:1000

Anti-Cyclin T2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111944

GTX111944 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 Xenograft, using cyclin T2(GTX111944) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cyclin T2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111944

GTX111944 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using cyclin T2(GTX111944) antibody at 1:200 dilution.

Anti-Cyclin T2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111944

GTX111944 IHC-P Image

Cyclin T2 antibody [C2C3], C-term detects cyclin T2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Cyclin T2 antibody [C2C3], C-term (GTX111944) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169