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Cyclophilin 40 antibody

Anti-Cyclophilin 40 antibody used in IHC (Paraffin sections) (IHC-P). GTX104038
Anti-Cyclophilin 40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104038
Anti-Cyclophilin 40 antibody used in Western Blot (WB). GTX104038
Anti-Cyclophilin 40 antibody used in Western Blot (WB). GTX104038
Anti-Cyclophilin 40 antibody used in IHC (Paraffin sections) (IHC-P). GTX104038

Cat. No. GTX104038

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($399),
25 μl ($169)
See all Cyclophilin 40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclophilin 40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036710

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase D , CYP-40 , CYPD

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclophilin 40 antibody used in IHC (Paraffin sections) (IHC-P). GTX104038

GTX104038 IHC-P Image

Cyclophilin 40 antibody detects Cyclophilin 40 protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Cyclophilin 40 antibody (GTX104038) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cyclophilin 40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104038

GTX104038 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using Cyclophilin 40(GTX104038) antibody at 1:200 dilution.

Anti-Cyclophilin 40 antibody used in Western Blot (WB). GTX104038

GTX104038 WB Image

Sample (30 ug of whole cell lysate)

A: Hep G2 (GTX27900)

10% SDS PAGE

GTX104038 diluted at 1:1000

Anti-Cyclophilin 40 antibody used in Western Blot (WB). GTX104038

GTX104038 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX104038 diluted at 1:1000

Anti-Cyclophilin 40 antibody used in IHC (Paraffin sections) (IHC-P). GTX104038

GTX104038 IHC-P Image

Cyclophilin 40 antibody detects PPID protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Cyclophilin 40 antibody (GTX104038) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Rosales Ramirez R et al. J Virol 2019; 93 (4) The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex.
Romel Rosales Ramirez et al. bioRxiv 2018; (Epub) The dengue virus non-structural protein 1 (NS1) is secreted from mosquito cells in association with the intracellular cholesterol transporter chaperone caveolin complex.
Application : ICC/IF
Reactivity : Hamster
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169