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Cyclophilin A antibody [C1C3]

KO/KD Orthogonal
GTX104698_40268_20180824_WB_shRNA_watermark_19011506_739.jpg
GTX104698_39638_IFA_19011506_807.jpg
GTX104698_39638_20150119_WB_Fraction_19011506_893.jpg
GTX104698_40268_WB_19011506_235.jpg
GTX104698_IHC_19011506_113.jpg
GTX104698_40268_20150313_WB_M_brain_19011506_791.jpg

Cat No. GTX104698

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HepG2 , Raji WCE , Raji C+M , Raji NE , mouse brain

Predict Reactivity

Rat, Sheep, Cat, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cyclophilin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CYPA antibody, CYPH antibody, MGC117158 antibody, MGC12404 antibody, MGC23397 antibody, PPIA antibody, cyclophilin antibody, PPIase A antibody, rotamase A antibody, peptidyl-prolyl cis-trans isomerase A antibody, cyclosporin A-binding protein antibody, T cell cyclophilin antibody, peptidylprolyl isomerase A (cyclophilin A) antibody

Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq]

Database

DATA IMAGES
GTX104698_40268_20180824_WB_shRNA_watermark_19011506_739.jpg

GTX104698 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [C1C3] (GTX104698) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104698_39638_IFA_19011506_807.jpg

GTX104698 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cyclophilin A(GTX104698) antibody at 1:200 dilution.

GTX104698_39638_20150119_WB_Fraction_19011506_893.jpg

GTX104698 WB Image

Orthogonal

Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX104698) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104698_40268_WB_19011506_235.jpg

GTX104698 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
GTX104698 diluted at 1:3000

GTX104698_IHC_19011506_113.jpg

GTX104698 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Cyclophilin A(GTX104698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104698_40268_20150313_WB_M_brain_19011506_791.jpg

GTX104698 WB Image

Cyclophilin A antibody detects Cyclophilin A protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX104698) at a dilution of 1:20000.

REFERENCE
REVIEW

Cyclophilin A antibody [C1C3] Cat No. GTX104698

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC3 cells
Amount used : 80 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169