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Cyclophilin B antibody [k2E2]

Anti-Cyclophilin B antibody [k2E2] used in Western Blot (WB). GTX50046

Cat No. GTX50046

Host

Mouse

Clonality

Monoclonal

Clone Name

k2E2

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Cyclophilin B (26-216aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Peptidylprolyl Isomerase B , B , Cyp-S1 , Cypb , Hel-S-39 , Oi9 , Scylp , Ppib

Cellular Localization

Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Background

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin.

Database

Research Area

DATA IMAGES

Anti-Cyclophilin B antibody [k2E2] used in Western Blot (WB). GTX50046

GTX50046 WB Image

Cell lysates of HepG2(30ug) and HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf