GeneTex
  • Country / Location Selection

United States (US)

Cyclophilin F antibody [AT1F5]

Anti-Cyclophilin F antibody [AT1F5] used in Western Blot (WB). GTX50026
Anti-Cyclophilin F antibody [AT1F5] used in IHC (Paraffin sections) (IHC-P). GTX50026

Cat No. GTX50026

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1F5

Isotype

IgG2b

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot is 1:500~1:1,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Cyclophilin F (30-207aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Peptidylprolyl Isomerase F , Cyp3 , Cyp-M , Cyp-D , Cypd , Ppif

Background

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-transisomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Database

Research Area

DATA IMAGES

Anti-Cyclophilin F antibody [AT1F5] used in Western Blot (WB). GTX50026

GTX50026 WB Image

Cell lysates of 293T and HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-Cyclophilin F antibody [AT1F5] used in IHC (Paraffin sections) (IHC-P). GTX50026

GTX50026 IHC-P Image

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

REFERENCE

There are currently no references for Cyclophilin F antibody [AT1F5] (GTX50026). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cyclophilin F antibody [AT1F5] (GTX50026). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf