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Cyclophilin F antibody [AT1F5]

Anti-Cyclophilin F antibody [AT1F5] used in IHC (Paraffin sections) (IHC-P). GTX50026
Anti-Cyclophilin F antibody [AT1F5] used in Western Blot (WB). GTX50026

Cat. No. GTX50026

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1F5

Isotype

 IgG2b

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Cyclophilin F products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot is 1:500~1:1,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Cyclophilin F (30-207aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase F , CYP3 , CyP-M , Cyp-D , CypD

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclophilin F antibody [AT1F5] used in IHC (Paraffin sections) (IHC-P). GTX50026

GTX50026 IHC-P Image

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Anti-Cyclophilin F antibody [AT1F5] used in Western Blot (WB). GTX50026

GTX50026 WB Image

Cell lysates of 293T and HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399