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Cyclophilin F antibody [N1C3]

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951
Anti-Cyclophilin F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117951

Cat No. GTX117951

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A431 , mouse heart , rat heart

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cyclophilin F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170163

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peptidylprolyl isomerase F , CYP3 , CyP-M , Cyp-D , CypD

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Wild-type (WT) and Cyclophilin F knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117951

GTX117951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PPIF(GTX117951) antibody at 1:500 dilution.

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in Western Blot (WB). GTX117951

GTX117951 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin F antibody (GTX117951) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclophilin F antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117951

GTX117951 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPIF(GTX117951) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169