GeneTex
United States (US)

Cystatin 8 (aa 23-34) antibody, Internal

GTX88202_2673_WB_18121411_357.jpg

Cat No. GTX88202

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88202-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-DPKKNETGVLRK, from the internal region of the protein sequence according to NP_005483.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CST8 Antibody , CRES Antibody , CTES5 Antibody

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing identified in mouse is suggested in human based on EST evidence but the full-length nature of putative variants has not been determined. [provided by RefSeq, Jul 2008
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Database

Research Area

DATA IMAGES
GTX88202_2673_WB_18121411_357.jpg

GTX88202 WB Image

WB analysis of HEK293 overexpressing human Cystatin 8 with C-terminal MYC tag using GTX88202 Cystatin 8 (aa 23-34) antibody, Internal.
Lane A : primary antibody
Lane B : Mock-transfected HEK293 probed with primary antibody
Lane C : Tag antibody
Dilution : 0.3µg/ml
Loading : 10µg protein in RIPA buffer