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Cystatin A antibody

Anti-Cystatin A antibody used in Western Blot (WB). GTX130885
Anti-Cystatin A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130885

Cat No. GTX130885

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cystatin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886376

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cystatin A , AREI , PSS4 , STF1 , STFA

Cellular Localization

Cytoplasm

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cystatin A antibody used in Western Blot (WB). GTX130885

GTX130885 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin A antibody (GTX130885) diluted at 1:2500.

Anti-Cystatin A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130885

GTX130885 ICC/IF Image

Cystatin A antibody detects Cystatin A protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cystatin A stained by Cystatin A antibody (GTX130885) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169