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Cystatin A antibody

Anti-Cystatin A antibody used in Western Blot (WB). GTX53962
Anti-Cystatin A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53962

Cat No. GTX53962

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTA (NP_005204.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cystatin A , AREI , PSS4 , STF1 , STFA

Cellular Localization

Cytoplasm

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cystatin A antibody used in Western Blot (WB). GTX53962

GTX53962 WB Image

WB analysis of THP-1 cell lysate using GTX53962 Cystatin A antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Cystatin A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53962

GTX53962 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX53962 Cystatin A antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf