GeneTex
United States (US)

Cystatin B antibody, C-term

GTX15970_1425_WB_18121410_718.jpg
GTX15970_403_IHC-P_18121410_699.jpg

Cat No. GTX15970

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX15970-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QTNKAKHDELTYF, from the C Terminus of the protein sequence according to NP_000091.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cystatin B,Cpi-B,Cst6,Epm1,Epm1A,Pme,Stfb,Uld,Cstb

Cellular Localization

Cytoplasmic and Nuclear

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX15970_1425_WB_18121410_718.jpg

GTX15970 WB Image

WB analysis of human lungs using GTX15970 Cystatin B antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX15970_403_IHC-P_18121410_699.jpg

GTX15970 IHC-P Image

IHC-P analysis of human tonsil using GTX15970 Cystatin B antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Package List Price ($)
$ 329