Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cystatin F antibody (GTX100613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Cystatin F antibody detects Cystatin F protein at vesicle by immunofluorescent analysis.
Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cystatin F stained by Cystatin F antibody (GTX100613) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using cystatin F(GTX100613) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min