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Cytochrome C antibody [4B10]

Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345
Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345
Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345
Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345
Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345
Anti-Cytochrome C antibody [4B10] used in Western Blot (WB). GTX34345

Cat. No. GTX34345

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B10

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cytochrome C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
IHC-P 1:500-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Protein

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CYC,CYCS,HCS,THC4,cytochrome c, somatic,Cytochrome C

Cellular Localization

Mitochondrion intermembrane space

Background

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345

GTX34345 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX34345 Cytochrome C antibody [4B10].

Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345

GTX34345 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX34345 Cytochrome C antibody [4B10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345

GTX34345 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX34345 Cytochrome C antibody [4B10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345

GTX34345 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34345 Cytochrome C antibody [4B10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Cytochrome C antibody [4B10] used in IHC (Paraffin sections) (IHC-P). GTX34345

GTX34345 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34345 Cytochrome C antibody [4B10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-Cytochrome C antibody [4B10] used in Western Blot (WB). GTX34345

GTX34345 WB Image

WB analysis of various sample lysates using GTX34345 Cytochrome C antibody [4B10].
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : Mouse liver tissue lysate
Lane 5 : Rat liver tissue lysate
Lane 5 : Rat kidney tissue lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399