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Cytochrome C antibody [7H8.2C12]

Anti-Cytochrome C antibody [7H8.2C12] used in Flow cytometry (FACS). GTX13575
Anti-Cytochrome C antibody [7H8.2C12] used in Western Blot (WB). GTX13575
Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX13575
Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX13575

Cat. No. GTX13575

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7H8.2C12

Isotype

 IgG2b

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Drosophila, Dog, Frog, Horse, pigeon
Package
100 μg ($529)
See all Cytochrome C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Product Note

This antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Purification

Protein A/G purified

Conjugation

Unconjugated

RRID

AB_369864

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c, somatic , CYC , HCS , THC4

Background

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody [7H8.2C12] used in Flow cytometry (FACS). GTX13575

GTX13575 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX13575 Cytochrome C antibody [7H8.2C12].
Blue : Primary antibody
Red : Isotype control

Anti-Cytochrome C antibody [7H8.2C12] used in Western Blot (WB). GTX13575

GTX13575 WB Image

WB analysis of heart tissue lysate using GTX13575 Cytochrome C antibody [7H8.2C12].

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX13575

GTX13575 IHC-P Image

IHC-P analysis of human salivary tumor tissue using GTX13575 Cytochrome C antibody [7H8.2C12].

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX13575

GTX13575 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX13575 Cytochrome C antibody [7H8.2C12].

REFERENCE

Application Reference

Wu TH et al. Acta Neuropathol 2013; 125 (5) : 711-25 Loss of vesicular * release precedes tauopathy in degenerative dopaminergic neurons in a Drosophila model expressing human tau.
Application : WB
Reactivity : Drosophila
Giri? M et al. J Surg Res 2010; 164 (2) : 266-75 Heme oxygenase-1 prevents hyperthyroidism induced hepatic damage via an antioxidant and antiapoptotic pathway.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529