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Cytochrome C antibody [7H8.2C12]

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622
Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622

Cat No. GTX34622

Host

Mouse

Clonality

Monoclonal

Clone Name

7H8.2C12

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila, Dog, Frog, Horse, pigeon
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Specificity/Sensitivity

It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

PROPERTIES

Form

Liquid

Buffer

1mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cytochrome C, Somatic , Cyc , Hcs , Thc4 , Cycs

Background

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622

GTX34622 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreas stained with Cytochrome C Monoclonal Antibody (7H8.2C12).

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622

GTX34622 IHC-P Image

Formalin-fixed, paraffin-embedded human Salivary Tumor stained with Cytochrome C Monoclonal Antibody (7H8.2C12).

REFERENCE

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REVIEW

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Package List Price ($)
$ 429