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Cytochrome C antibody [7H8.2C12]

Anti-Cytochrome C antibody [7H8.2C12] used in Western Blot (WB). GTX34622
Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622
Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622
Anti-Cytochrome C antibody [7H8.2C12] used in Flow cytometry (FACS). GTX34622

Cat No. GTX34622

Host

Mouse

Clonality

Monoclonal

Clone Name

7H8.2C12

Isotype

IgG2b

Application

WB, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Drosophila, Dog, Frog, Horse, pigeon
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CYC , CYCS , HCS , THC4 , cytochrome c, somatic , Cytochrome C

Cellular Localization

Mitochondrion intermembrane space

Background

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody [7H8.2C12] used in Western Blot (WB). GTX34622

GTX34622 WB Image

WB analysis of heart tissue lysate using GTX34622 Cytochrome C antibody [7H8.2C12].

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622

GTX34622 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX34622 Cytochrome C antibody [7H8.2C12].

Anti-Cytochrome C antibody [7H8.2C12] used in IHC (Paraffin sections) (IHC-P). GTX34622

GTX34622 IHC-P Image

IHC-P analysis of human salivary tumor tissue using GTX34622 Cytochrome C antibody [7H8.2C12].

Anti-Cytochrome C antibody [7H8.2C12] used in Flow cytometry (FACS). GTX34622

GTX34622 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX34622 Cytochrome C antibody [7H8.2C12].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339