Cytochrome C antibody [CTC05]

Cat. No. GTX34620

Host	Mouse
Clonality	Monoclonal
Clone Name	CTC05
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Drosophila, Dog, Frog, Horse, pigeon
Package	100 μg ($529)

See all Cytochrome C products

Datasheet
Document

View product citations for antibody GTX34620 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	0.25-0.5μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant cytochrome c protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CYC , CYCS , HCS , THC4 , cytochrome c, somatic , Cytochrome C

Cellular Localization

Mitochondrion intermembrane space

Background

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Database

Gene ID: 54205 CYCS
UniProt: P99999 CYCS

Research Area

DATA IMAGES

GTX34620 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34620 Cytochrome C antibody [CTC05].
Green : Primary antibody
Blue : nucleus

GTX34620 IHC-P Image

IHC-P analysis of human heart tissue using GTX34620 Cytochrome C antibody [CTC05].

GTX34620 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX34620 Cytochrome C antibody [CTC05].
Blue : Primary antibody
Red : Isotype control

GTX34620 WB Image

WB analysis of heart tissue lysate using GTX34620 Cytochrome C antibody [CTC05].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty