GeneTex
United States (US)

Cytochrome C antibody [GT577]

GTX633691_42632_20161027_WB_18121411_362.jpg
GTX633691_42632_20161124_WB_18121411_299.jpg
GTX633691_42632_20161219_IHC-P_R_18121411_650.jpg
GTX633691_42632_20161219_IHC-P_M_18121411_930.jpg

Cat No. GTX633691

Host Mouse
Clonality Monoclonal
Clone Name GT577
Isotype IgA
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

K562 , HeLa

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.64mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cytochrome C recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CYC antibody , CYCS antibody , Cytochrome C antibody , HCS antibody , THC4 antibody , cytochrome c, somatic antibody

Cellular Localization

Mitochondrion matrix

Background

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX633691_42632_20161027_WB_18121411_362.jpg

GTX633691 WB Image

Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [GT577] (GTX633691) diluted at 1:500.

GTX633691_42632_20161124_WB_18121411_299.jpg

GTX633691 WB Image

Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX633691) diluted at 1:3000.

GTX633691_42632_20161219_IHC-P_R_18121411_650.jpg

GTX633691 IHC-P Image

Cytochrome C antibody [GT577] detects Cytochrome C protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Cytochrome C antibody [GT577] (GTX633691) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633691_42632_20161219_IHC-P_M_18121411_930.jpg

GTX633691 IHC-P Image

Cytochrome C antibody [GT577] detects Cytochrome C protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Cytochrome C antibody [GT577] (GTX633691) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169