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Cytochrome C antibody [GT577]

Anti-Cytochrome C antibody [GT577] used in IHC (Paraffin sections) (IHC-P). GTX633691
Anti-Cytochrome C antibody [GT577] used in Western Blot (WB). GTX633691
Anti-Cytochrome C antibody [GT577] used in IHC (Paraffin sections) (IHC-P). GTX633691
Anti-Cytochrome C antibody [GT577] used in Western Blot (WB). GTX633691

Cat. No. GTX633691

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT577

Isotype

 IgA

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cytochrome C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

HeLa , K562

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cytochrome C recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2888367

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c, somatic , CYC , HCS , THC4

Cellular Localization

Mitochondrion matrix

Background

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody [GT577] used in IHC (Paraffin sections) (IHC-P). GTX633691

GTX633691 IHC-P Image

Cytochrome C antibody [GT577] detects Cytochrome C protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Cytochrome C antibody [GT577] (GTX633691) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytochrome C antibody [GT577] used in Western Blot (WB). GTX633691

GTX633691 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX633691) diluted at 1:3000.

Anti-Cytochrome C antibody [GT577] used in IHC (Paraffin sections) (IHC-P). GTX633691

GTX633691 IHC-P Image

Cytochrome C antibody [GT577] detects Cytochrome C protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Cytochrome C antibody [GT577] (GTX633691) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytochrome C antibody [GT577] used in Western Blot (WB). GTX633691

GTX633691 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [GT577] (GTX633691) diluted at 1:500.

REFERENCE

Application Reference

Yang D et al. Int Immunopharmacol 2021; 90 : 107149 Mitoquinone ameliorates cigarette smoke-induced airway inflammation and mucus hypersecretion in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations