GeneTex
United States (US)

Cytochrome C antibody [SJL2-4(apo)]

img25837_18121411_760.jpg
Cytochrome-C-antibody-SJL2-4apo_WB_GTX16076-1_18121409_803.jpg

Cat No. GTX16076

Host Mouse
Clonality Monoclonal
Clone Name SJL2-4(apo)
Isotype IgG2b
Application WB
Reactivity Human, Mouse, Rat, Goat, Sheep, Monkey
APPLICATION

Application Note

WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 12 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

12 kDa. ( Note )

Positive Control

293T cells
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Horse).

Purification

>95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome C, Somatic,Cyc,Hcs,Thc4,Cycs

Cellular Localization

Cytoplasmic and Mitochondrial

Background

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

Database

Research Area

DATA IMAGES
img25837_18121411_760.jpg

GTX16076 WB Image

Detection of cytochrome c in human cells. Lane 1: cytoplasmic fraction, Lane 2: nuclear fraction. Cytochrome c migrates as a 13 kDa species.

Cytochrome-C-antibody-SJL2-4apo_WB_GTX16076-1_18121409_803.jpg

GTX16076 WB Image

Detection of cytochrome c in human cells. Cytochrome c migrates as a 13 kDa species
Lane 1: cytoplasmic fraction
Lane 2: nuclear fraction

Package List Price ($)
$ 319