Cytochrome C antibody

GTX108585_42809_20180817_WB_shRNA_watermark_19011507_158.jpg

Cat No. GTX108585

Reference 13

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat

See all Cytochrome C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

293T , K562 , THP-1 , HL-60 , Mouse brain , PC-12 , CYCS shRNA-transfected 293T , *293T , *SCC-25

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cytochrome C Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CYC antibody, HCS antibody, THC4 antibody, CYCS antibody, cytochrome c antibody, "cytochrome c, somatic antibody"

Cellular Localization

Mitochondrion matrix

Background

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

Database

Gene ID:54205
UniProt:P99999

Research Area

DATA IMAGES

GTX108585 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108585 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 25599133

GTX108585 IHC-P Image

Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108585 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108585 IHC-P Image

Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108585 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE