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Cytochrome C antibody

KO/KD Orthogonal
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Anti-Cytochrome C antibody used in IHC (Paraffin sections) (IHC-P). GTX108585
Anti-Cytochrome C antibody used in IHC (Paraffin sections) (IHC-P). GTX108585

Cat. No. GTX108585

Reference 33
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Drosophila, Dog
Package
100 μl ($399),
25 μl ($169)
See all Cytochrome C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Mouse brain , PC-12 , K562 , THP-1 , HL-60 , 293T

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cytochrome C Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036712

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c, somatic , CYC , HCS , THC4

Cellular Localization

Mitochondrion matrix

Background

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (GTX108585) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585

GTX108585 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
15% SDS PAGE
GTX108585 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytochrome C antibody used in IHC (Paraffin sections) (IHC-P). GTX108585

GTX108585 IHC-P Image

Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytochrome C antibody used in IHC (Paraffin sections) (IHC-P). GTX108585

GTX108585 IHC-P Image

Cytochrome C antibody detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Cytochrome C stained by Cytochrome C antibody (GTX108585) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ogasawara A et al. Fujita Med J 2023; 9 (2) : 147-153 Agaritine derived from Agaricus blazei Murrill induces apoptosis via mitochondrial membrane depolarization in hematological tumor cell lines.
Garc?a-Arroyo R et al. Int J Mol Sci 2022; 23 (19) CERKL, a Retinal Dystrophy Gene, Regulates Mitochondrial Transport and Dynamics in Hippocampal Neurons.
Application : WB
Reactivity : Mouse
Wang CH et al. J Am Heart Assoc 2022; 11 (21) : e027685 Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia-Induced Cardiac Arrest and Resuscitation Rat Model.
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Huang FL et al. Oncol Rep 2022; 47 (2) Niclosamide suppresses T?cell acute lymphoblastic leukemia growth through activation of apoptosis and autophagy.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Cytochrome C antibody Cat. No. GTX108585

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28928421
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Date : Anonymous submitted on 10-Jan-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC-25 cells
Amount used : 25μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Cytochrome C antibody used in Western Blot (WB). GTX108585
Date : Anonymous submitted on 07-May-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 16Hr
Submit a Review
Application Reference
Ogasawara A et al. Fujita Med J 2023; 9 (2):147-153 Agaritine derived from Agaricus blazei Murrill induces apoptosis via mitochondrial membrane depolarization in hematological tumor cell lines.
Garc?a-Arroyo R et al. Int J Mol Sci 2022; 23 (19) CERKL, a Retinal Dystrophy Gene, Regulates Mitochondrial Transport and Dynamics in Hippocampal Neurons.
Application : WB
Reactivity : Mouse
Wang CH et al. J Am Heart Assoc 2022; 11 (21):e027685 Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia-Induced Cardiac Arrest and Resuscitation Rat Model.
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Huang FL et al. Oncol Rep 2022; 47 (2) Niclosamide suppresses T?cell acute lymphoblastic leukemia growth through activation of apoptosis and autophagy.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Erin Clough et al. J Neuroimmune Pharmacol 2021; 16 (4):770-784 Mitochondrial Dynamics in SARS-COV2 Spike Protein Treated Human Microglia: Implications for Neuro-COVID.
Application : ICC/IF
Reactivity : Human
Hongzhi Hu et al. J Nanobiotechnology 2021; 19 (1):390 Mitochondria-targeted accumulation of oxygen-irrelevant free radicals for enhanced synergistic low-temperature photothermal and thermodynamic therapy.
Application : WB
Reactivity : Human
Yang DQ et al. Oxid Med Cell Longev 2021; 2021:6644238 Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke.
Application : WB
Liao MF et al. Biomedicines 2021; 9 (5) Interactions between Autophagy, Proinflammatory Cytokines, and Apoptosis in Neuropathic Pain: Granulocyte Colony Stimulating Factor as a Multipotent Therapy in Rats with Chronic Constriction Injury.
Application : WB
Reactivity : Rat
Kunzke T et al. J Cachexia Sarcopenia Muscle 2020; 11 (1):226-240 Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.
Cornelissen T et al. Hum Mol Genet 2020; (Epub) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2.
Application : WB
Reactivity : Human
Thomas Kunzke et al. Journal of Cachexia, Sarcopenia and Muscle 2020; (Epub) Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.
Application : IHC-P
Reactivity : Mouse
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Romero MA et al. Am J Physiol Cell Physiol 2019; (Epub) Five months of voluntary wheel running downregulates skeletal muscle LINE-1 gene expression in rats.
Application : WB
Reactivity : Rat
Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.
Application : WB
Reactivity : Human
Huang SH et al. Phytomedicine 2019; 64:153068 Rooibos suppresses proliferation of castration-resistant prostate cancer cells via inhibition of Akt signaling.
Application : WB
Reactivity : Human
Kuriyama S et al. Cell Death Discov 2018; 4:11 PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Application : WB
Reactivity : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
Application : WB
Reactivity : Human
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Sharikova AV et al. J Neuroimmune Pharmacol 2018; (Epub)
Application : WB
Reactivity : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
Kuriyama S et al. Cell Death Discovery 2018; (Epub) PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer.
Application : WB
Reactivity : Human
Wang JP et al. Oncol Lett 2017; 14 (3):3503-3509 Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells.
Li SM et al. Sci Rep 2017; 7 (1):11893 Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma.
Application : WB
Reactivity : Human
Tsui KH et al. Reproduction 2017; 154 (2):101-110 DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
Application : WB
Reactivity : Human
Nehra S et al. High Alt Med Biol 2016; Chronic Hypobaric Hypoxia Induces Right Ventricular Hypertrophy and Apoptosis in Rats: Therapeutic Potential of Nanocurcumin in Improving Adaptation.
Application : IHC-Fr
Reactivity : Rat
Chiang MC et al. Exp Cell Res 2016; 347 (2):322-31 * activation of ampk-dependent pathways is neuroprotective in human neural stem cells against amyloid-beta-induced mitochondrial dysfunction.
Application : WB
Reactivity : Human
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB
Reactivity : Human
Ruan Y et al. Mol Med Rep 2015; 12 (4):5796-806 Autophagy inhibition enhances isorhamnetin-induced mitochondria-dependent apoptosis in non-small cell lung cancer cells.
Li CJ et al. Sci Rep 2015; 24 (5):9819 Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169