Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , K562 , THP-1 , HL-60 , Mouse brain , PC-12 , CYCS shRNA-transfected 293T , *293T , *SCC-25
Predict Reactivity
Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.57mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Cytochrome C Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
CYC antibody, HCS antibody, THC4 antibody, CYCS antibody, cytochrome c antibody, "cytochrome c, somatic antibody"
Cellular Localization
Mitochondrion matrix
Background
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]
Database
Research Area