ICC/IF analysis of HepG2 cells using GTX15837 Cytochrome P450 3A5 antibody [F18 P3 B6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of whole cell extracts (30 μg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4) and Mouse Kidney (Lane 5) using GTX15837 Cytochrome P450 3A5 antibody [F18 P3 B6].
Dilution : 1:100-1:1000