GeneTex
United States (US)

Cytochrome P450 3A5 antibody [F18 P3 B6]

GTX15837_1548_WB_19011519_192.jpg
GTX15837_341_ICC-IF_19011519_600.jpg

Cat No. GTX15837

Host Mouse
Clonality Monoclonal
Clone Name F18 P3 B6
Isotype IgG1
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human, Mouse, Rat, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:250
IHC-P 1-5 μg/ml
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Monkey

Immunogen

Synthetic peptide corresponding to residues E S R D G T L S G E, conjugated to ovalbumin.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome P450 Family 3 Subfamily A Member 5,Cp35,Cypiiia5,P450Pcn3,Pcn3,Cyp3A5

Cellular Localization

Endoplasmic reticulum membrane,Microsome membrane

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES
GTX15837_1548_WB_19011519_192.jpg

GTX15837 WB Image

WB analysis of whole cell extracts (30 µg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Brain (Lane 3), Rat Brain (Lane 4) and Mouse Kidney (Lane 5) using GTX15837 Cytochrome P450 3A5 antibody [F18 P3 B6].
Dilution : 1:100-1:1000

GTX15837_341_ICC-IF_19011519_600.jpg

GTX15837 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX15837 Cytochrome P450 3A5 antibody [F18 P3 B6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Package List Price ($)
$ 319