APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:200-1:2000 |
| 1:20-1:200 |
| 1:100-1:500 |
| 1:100 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody detects cytochrome P450 4A (isoforms 1, 2, and 3).
PROPERTIES
Form
Liquid
Buffer
Serum diluted with PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Rat
Immunogen
Synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_11165164
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome P450, family 4, subfamily a, polypeptide 1 , CYPIVA10 , Cyp4a10 , Cyp4a22
Background
arachidonic acid monooxygenase; catalyzes the hydroxylation of omega-terminal carbon of the arachidonic acid [RGD, Feb 2006]
Database
DATA IMAGES
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GTX79175 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX79175 Cytochrome P450 4A antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
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GTX79175 ICC/IF Image
ICC/IF analysis of PC-12 cells using GTX79175 Cytochrome P450 4A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX79175 ICC/IF Image
ICC/IF analysis of H-4-II-E cells using GTX79175 Cytochrome P450 4A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX79175 IHC-P Image
IHC-P analysis of rat liver tissue using GTX79175 Cytochrome P450 4A antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
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GTX79175 WB Image
WB analysis of 25 ug of rat liver (Lane 1), H-4-II-E (Lane 2) and Hela (Lane 3) cell lysates using GTX79175 Cytochrome P450 4A antibody.
Dilution : 1:1000
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GTX79175 IHC-P Image
IHC-P analysis of human liver tissue using GTX79175 Cytochrome P450 4A antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200
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GTX79175 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX79175 Cytochrome P450 4A antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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REFERENCE
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REVIEW
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