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Cytochrome P450 4F2 antibody [N2C2], Internal

Anti-Cytochrome P450 4F2 antibody [N2C2], Internal used in Western Blot (WB). GTX102722

Cat. No. GTX102722

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Pig
Package
100 μl ($399),
25 μl ($169)
See all Cytochrome P450 4F2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HeLa cell membrane

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytochrome P450 4F2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome P450 family 4 subfamily F member 2 , CPF2

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytochrome P450 4F2 antibody [N2C2], Internal used in Western Blot (WB). GTX102722

GTX102722 WB Image

Sample (20 ug )
A: Hela whole cell lysate
B: Hela cell membrane
7.5% SDS PAGE
GTX102722 diluted at 1:1000

REFERENCE

Application Reference

Podszun MC et al. Toxicol Appl Pharmacol 2013; 266 (3) : 452-8 high-dose supplementation with natural α-tocopherol does neither alter the pharmacodynamics of * nor its phase i metabolism in guinea pigs.
Application : WB
Reactivity : Pig
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169