APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
CYB5A transfected 293T
Predict Reactivity
Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cytochrome b5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240669
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome b5 type A , CYB5 , MCB5 , METAG
Cellular Localization
Isoform 1: Endoplasmic reticulum membrane , Microsome membrane , Isoform 2: Cytoplasm
Background
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/templates/default/js/lightbox/images/loading.gif "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")
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GTX101909 WB Image
Western Blot analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody.
A: Non-transfected lysate.
B: CYB5A transfected lysate.
12% SDS PAGE
GTX101909 diluted at 1:500
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GTX101909 ICC/IF Image
Cytochrome b5 antibody detects Cytochrome b5 protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytochrome b5 stained by Cytochrome b5 antibody (GTX101909) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX101909 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 antibody (GTX101909) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX101909 ICC/IF Image
Cytochrome b5 antibody detects Cytochrome b5 protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cytochrome b5 stained by Cytochrome b5 antibody (GTX101909) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX101909 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 antibody (GTX101909) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Cytochrome b5 antibody [N1C3] (GTX101909). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytochrome b5 antibody [N1C3] (GTX101909). Be the first to share your experience with this product.
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/upload/website/prouct_img/small/GTX101909/GTX101909_WB_w_23060100_309.webp "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909](/upload/website/prouct_img/small/GTX101909/GTX101909_39694_20190612_ICC_IF_D_22111423_262.webp "Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/upload/website/prouct_img/small/GTX101909/GTX101909_39694_20190322_WB_C_22111423_564.webp "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909](/upload/website/prouct_img/small/GTX101909/GTX101909_39694_20190612_ICC_IF_C_22111423_504.webp "Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/upload/website/prouct_img/small/GTX101909/GTX101909_39694_20190201_WB_D_22111423_223.webp "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/upload/website/prouct_img/normal/GTX101909/GTX101909_WB_w_23060100_309.webp "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909](/upload/website/prouct_img/normal/GTX101909/GTX101909_39694_20190612_ICC_IF_D_22111423_262.webp "Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/upload/website/prouct_img/normal/GTX101909/GTX101909_39694_20190322_WB_C_22111423_564.webp "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909](/upload/website/prouct_img/normal/GTX101909/GTX101909_39694_20190612_ICC_IF_C_22111423_504.webp "Anti-Cytochrome b5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101909")
![Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909](/upload/website/prouct_img/normal/GTX101909/GTX101909_39694_20190201_WB_D_22111423_223.webp "Anti-Cytochrome b5 antibody [N1C3] used in Western Blot (WB). GTX101909")