GeneTex
United States (US)

Cytochrome b5 antibody [N1C3]

GTX101909_WB_19011506_295.jpg

Cat No. GTX101909

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Cytochrome b5 tansfected 293T cell

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytochrome b5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CYB5 antibody, MCB5 antibody, CYB5A antibody, type 1 cyt-b5 antibody, cytochrome b5 antibody, cytochrome b5 type A (microsomal) antibody

Cellular Localization

Isoform 1: Endoplasmic reticulum membrane , Microsome membrane , Isoform 2: Cytoplasm

Background

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101909_WB_19011506_295.jpg

GTX101909 WB Image

Western Blot analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody.
A: Non-transfected lysate.
B: CYB5A transfected lysate.
12% SDS PAGE
GTX101909 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169