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Cytochrome b5 antibody

Anti-Cytochrome b5 antibody used in Western Blot (WB). GTX53959
Anti-Cytochrome b5 antibody used in IHC (Paraffin sections) (IHC-P). GTX53959
Anti-Cytochrome b5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53959
Anti-Cytochrome b5 antibody used in IHC (Paraffin sections) (IHC-P). GTX53959

Cat. No. GTX53959

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cytochrome b5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CYB5A (NP_683725.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome b5 type A , CYB5 , MCB5 , METAG

Background

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-Cytochrome b5 antibody used in Western Blot (WB). GTX53959

GTX53959 WB Image

WB analysis of various sample lysates using GTX53959 Cytochrome b5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Cytochrome b5 antibody used in IHC (Paraffin sections) (IHC-P). GTX53959

GTX53959 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX53959 Cytochrome b5 antibody.
Dilution : 1:100

Anti-Cytochrome b5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53959

GTX53959 ICC/IF Image

ICC/IF analysis of C6 cells using GTX53959 Cytochrome b5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Cytochrome b5 antibody used in IHC (Paraffin sections) (IHC-P). GTX53959

GTX53959 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX53959 Cytochrome b5 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399