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Cytochrome c Release Assay Kit

Cytochrome c Release Assay Kit. GTX85531

Cat No. GTX85531

Application

WB
Package
1 kit ($669)
PRODUCT

Summary

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. GeneTex’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.



‧ Detection method- Western blotting

‧ Sample type- Cells and Tissues

‧ Species reactivity- Mammalian

‧ Kit size- 100 assays

‧ Applications- Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.


Features and Benefits

‧ Simple procedure; takes ~3 hours

‧ Fast and convenient

‧ Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required. The kit provides optimal reagents and antibodies for 100 assays



Kit Contents:

Mitochondria Extraction Buffer

5X Cytosol Extraction Buffer

DTT (1 M)

500X Protease Inhibitor Cocktail (Lyophilized): 1 vial - Add 250 μl DMSO to dissolve the 500X Protease Inhibitor Cocktail before use

Anti-Cytochrome c Mouse mAb (0.2 mg/ml)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitochondrial Cytochrome c Release Assay , Apoptosis Assay Kit

Research Area

DATA IMAGES

Cytochrome c Release Assay Kit. GTX85531

GTX85531 Image

Cytochrome c Was Released from Mitochondria into Cytosol in Apoptotic Cells. Apoptosis was induced in Jurkat cells with camptothecin. Mitochondria and cytosol fractions were isolated at 6 hours following induction of apoptosis and probed with cytochrome c antibody according to the kit instructions. Mito = Mitochondria; Cyto = Cytosol.

REFERENCE

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