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Cytohesin 1 + Cytohesin 2 antibody [6G11]

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in IHC (Paraffin sections) (IHC-P). GTX22729
Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22729
Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in IHC (Paraffin sections) (IHC-P). GTX22729
Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22729
Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22729
Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in IHC (Paraffin sections) (IHC-P). GTX22729

Cat. No. GTX22729

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Cytohesin 1,2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1.0 μg/ml
ICC/IF 1:100
IHC-P 1:200-500
IP Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified, His-tagged cytohesin-1.

Purification

Purified by polyethylene glycol precipitation

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytohesin 2 , ARNO , CTS18 , CTS18.1 , PSCD2 , PSCD2L , SEC7L , Sec7p-L , Sec7p-like , cytohesin-2

Cellular Localization

Cell membrane,Cytoplasm

Background

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Database

DATA IMAGES

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in IHC (Paraffin sections) (IHC-P). GTX22729

GTX22729 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22729 Cytohesin 1 + Cytohesin 2 antibody [6G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:300

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22729

GTX22729 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX22729 Cytohesin 1 + Cytohesin 2 antibody [6G11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in IHC (Paraffin sections) (IHC-P). GTX22729

GTX22729 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22729 Cytohesin 1 + Cytohesin 2 antibody [6G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22729

GTX22729 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX22729 Cytohesin 1 + Cytohesin 2 antibody [6G11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22729

GTX22729 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22729 Cytohesin 1 + Cytohesin 2 antibody [6G11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Cytohesin 1 + Cytohesin 2 antibody [6G11] used in IHC (Paraffin sections) (IHC-P). GTX22729

GTX22729 IHC-P Image

IHC-P analysis of human placenta tissue using GTX22729 Cytohesin 1 + Cytohesin 2 antibody [6G11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399