ICC/IF analysis of HeLa cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of NIH-3T3 cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

WB analysis of whole cell extracts (30 μg lysate) of Raji (Lane 1), Jurkat (Lane 2) and Hep G2 (Lane 3) using GTX22727 Cytohesin 1 antibody [2E11].
Dilution : 0.5-2 μg/ml

ICC/IF analysis of HepG2 cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of HeLa cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature