APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1.0 μg/ml |
| 1:20 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified, His-tagged cytohesin-1.
Purification
Purified by polyethylene glycol precipitation
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytohesin 1 , B2-1 , CYTOHESIN-1 , D17S811E , PSCD1 , SEC7
Cellular Localization
Cell membrane,Cytoplasm, cytosol
Background
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/templates/default/js/lightbox/images/loading.gif "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
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GTX22727 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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GTX22727 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
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GTX22727 WB Image
WB analysis of whole cell extracts (30 μg lysate) of Raji (Lane 1), Jurkat (Lane 2) and Hep G2 (Lane 3) using GTX22727 Cytohesin 1 antibody [2E11].
Dilution : 0.5-2 μg/ml
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GTX22727 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
|
|
|
GTX22727 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22727 Cytohesin 1 antibody [2E11]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
|
REFERENCE
There are currently no references for Cytohesin 1 antibody [2E11] (GTX22727). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytohesin 1 antibody [2E11] (GTX22727). Be the first to share your experience with this product.
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/small/GTX22727/GTX22727_384_ICC-IF_w_23060620_110.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/small/GTX22727/GTX22727_387_ICC-IF_w_23060620_316.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Western Blot (WB). GTX22727](/upload/website/prouct_img/small/GTX22727/GTX22727_1576_WB_w_23060620_570.webp "Anti-Cytohesin 1 antibody [2E11] used in Western Blot (WB). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/small/GTX22727/GTX22727_385_ICC-IF_w_23060620_154.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/small/GTX22727/GTX22727_386_ICC-IF_w_23060620_527.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/normal/GTX22727/GTX22727_384_ICC-IF_w_23060620_110.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/normal/GTX22727/GTX22727_387_ICC-IF_w_23060620_316.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Western Blot (WB). GTX22727](/upload/website/prouct_img/normal/GTX22727/GTX22727_1576_WB_w_23060620_570.webp "Anti-Cytohesin 1 antibody [2E11] used in Western Blot (WB). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/normal/GTX22727/GTX22727_385_ICC-IF_w_23060620_154.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")
![Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727](/upload/website/prouct_img/normal/GTX22727/GTX22727_386_ICC-IF_w_23060620_527.webp "Anti-Cytohesin 1 antibody [2E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22727")