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Cytohesin 1 antibody [N1C1]

Anti-Cytohesin 1 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX102825
Anti-Cytohesin 1 antibody [N1C1] used in Western Blot (WB). GTX102825

Cat. No. GTX102825

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Cytohesin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

PSCD1 transfected 293T

Predict Reactivity

Mouse, Rat, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytohesin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytohesin 1 , B2-1 , CYTOHESIN-1 , D17S811E , PSCD1 , SEC7

Background

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytohesin 1 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX102825

GTX102825 IHC-Wm Image

Cyth 1 antibody detects Cyth 1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Cyth 1 antibody (GTX102825) dilution: 1:200.
Anti-Cytohesin 1 antibody [N1C1] used in Western Blot (WB). GTX102825

GTX102825 WB Image

Western Blot analysis of PSCD1 expression in transfected 293T cell line by PSCD1 polyclonal antibody.
A: Non-transfected lysate.
B: PSCD1 transfected lysate.
12% SDS PAGE
GTX102825 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169