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Cytokeratin 10 antibody [DE-K10]

Anti-Cytokeratin 10 antibody [DE-K10] used in IHC (Paraffin sections) (IHC-P). GTX34626

Cat. No. GTX34626

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DE-K10

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Cat, Dog
Package
100 μg ($529)
See all Cytokeratin 10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1-2μg/ml for 30 min at RT
IHC-Fr Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCIE , BIE , CK10 , EHK , K10 , KPP , KRT10 , keratin 10 , Cytokeratin 10

Background

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 10 antibody [DE-K10] used in IHC (Paraffin sections) (IHC-P). GTX34626

GTX34626 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX34626 Cytokeratin 10 antibody [DE-K10].

REFERENCE

Clone Reference

Furukawa S et al. J Toxicol Pathol. 2017; 30 (1):79-107 Databases for technical aspects of immunohistochemistry.
Application : IHC-P
Reactivity : Mouse
Neumann C et al. J Invest Dermatol. 2015; 135 (2):471-480 The δ-opioid receptor affects epidermal homeostasis via ERK-dependent inhibition of transcription factor POU2F3.
Application : WB
Reactivity : Human
Li B et al. Clin Immunol. 2011; 140 (3):244-59 Thymic microenvironment reconstitution after postnatal human thymus transplantation.
Application : IHC-P, IHC-Fr
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529