PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
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Not tested in other applications.
Calculated MW
Positive Control
Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.
Negative Control
Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-lenth human KRT10 protein.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
keratin 10 , BCIE , BIE , CK10 , EHK , K10 , KPP
Background
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")
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GTX04401 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX04401 Cytokeratin 10 antibody [MSVA-610M] HistoMAX™.
Absence of cytokeratin 10 staining in non keratinizing squamous epithelium of the esophagus.
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GTX04401 IHC-P Image
IHC-P analysis of human squamous cell carcinoma (SCC) from vulva tissue using GTX04401 Cytokeratin 10 antibody [MSVA-610M] HistoMAX™.
Strong Cytokeratin 10 immunostaining in a squamous cell carcinoma of the vulva. The basal cell layers are largely unstained.
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GTX04401 IHC-P Image
IHC-P analysis of human skin tissue using GTX04401 Cytokeratin 10 antibody [MSVA-610M] HistoMAX™.
Cytokeratin 10 is strongly positive in all suprabasal cell layers of the epidermis and in associated sebaceous glands and hair follicles. The basal cell layer remains strictly Cytokeratin 10 negative.
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REFERENCE
There are currently no references for Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ (GTX04401). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ (GTX04401). Be the first to share your experience with this product.
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/upload/website/prouct_img/small/GTX04401/GTX04401_20230728_IHC-P_295_23072723_627.webp "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/upload/website/prouct_img/small/GTX04401/GTX04401_20230728_IHC-P_35_23072722_861.webp "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/upload/website/prouct_img/small/GTX04401/GTX04401_20230728_IHC-P_165_23072722_979.webp "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/upload/website/prouct_img/normal/GTX04401/GTX04401_20230728_IHC-P_295_23072723_627.webp "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/upload/website/prouct_img/normal/GTX04401/GTX04401_20230728_IHC-P_35_23072722_861.webp "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")
![Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401](/upload/website/prouct_img/normal/GTX04401/GTX04401_20230728_IHC-P_165_23072722_979.webp "Anti-Cytokeratin 10 antibody [MSVA-610M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04401")