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Cytokeratin 13 antibody, C-term

Anti-Cytokeratin 13 antibody, C-term used in Western Blot (WB). GTX88529
Anti-Cytokeratin 13 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88529

Cat. No. GTX88529

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Tonsil shows strong layered, filamentous staining.

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88529-PEP).

Product Note

This antibody is expected to recognize the reported isoform a (NP_002265.2) only.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence according to NP_705694.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 13 , CK13 , K13 , WSN2

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 13 antibody, C-term used in Western Blot (WB). GTX88529

GTX88529 WB Image

WB analysis of human lung lysate using GTX88529 Cytokeratin 13 antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Cytokeratin 13 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88529

GTX88529 IHC-P Image

IHC-P analysis of human tonsil using GTX88529 Cytokeratin 13 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399