GeneTex
United States (US)

Cytokeratin 13 antibody, C-term

GTX88529_2262_WB_18121411_871.jpg
GTX88529_926_IHC-P_18121411_880.jpg

Cat No. GTX88529

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 2-4μg/ml

Note :

Human Tonsil shows strong layered, filamentous staining.
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88529-PEP).

Specificity/Sensitivity

This antibody is expected to recognize the reported isoform a (NP_002265.2) only.
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence according to NP_705694.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

K13 Antibody , KRT13 Antibody , CK13 Antibody

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]
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Database

DATA IMAGES
GTX88529_2262_WB_18121411_871.jpg

GTX88529 WB Image

WB analysis of human lung lysate using GTX88529 Cytokeratin 13 antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88529_926_IHC-P_18121411_880.jpg

GTX88529 IHC-P Image

IHC-P analysis of human tonsil using GTX88529 Cytokeratin 13 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Package List Price ($)
$ 329