Cytokeratin 14 antibody [AT13F4]

Cat. No. GTX57611

Host	Mouse
Clonality	Monoclonal
Clone Name	AT13F4
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($399)

See all Cytokeratin 14 products

Datasheet
Document

View product citations for antibody GTX57611 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Recommended starting dilution is 1:1000.
ICC/IF	Assay dependent
IHC-P	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT13F4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT16 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 14 , CK14 , EBS3 , EBS4 , K14 , KRT14 , NFJ , Cytokeratin 14

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3861 KRT14
UniProt: P02533 KRT14

Research Area

DATA IMAGES

GTX57611 ICC/IF Image

ICC/IF analysis of PC-3 cells using GTX57611 Cytokeratin 14 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57611 IHC-P Image

IHC-P analysis of human cervical cancer using GTX57611 Cytokeratin 14 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:200

GTX57611 FACS Image

FACS analysis of A431 cells using GTX57611 Cytokeratin 14 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

GTX57611 WB Image

WB analysis of various samples using GTX57611 Cytokeratin 14 antibody.
Lane 1 : Recombinant Human KRT14
Lane 2 : A431 whole cell lysate (40 μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty