APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
1-2μg/ml for 30 min at RT |
0.5-1μg/10⁶ cells in 0.1ml |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human KRT14 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CK14 , EBS3 , EBS4 , K14 , KRT14 , NFJ , keratin 14 , Cytokeratin 14
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX34630 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34630 Cytokeratin 14 antibody [KRT14/532].
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GTX34630 IHC-P Image
IHC-P analysis of human cervix tissue using GTX34630 Cytokeratin 14 antibody [KRT14/532].
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REFERENCE
There are currently no references for Cytokeratin 14 antibody [KRT14/532] (GTX34630). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 14 antibody [KRT14/532] (GTX34630). Be the first to share your experience with this product.