GeneTex
United States (US)

Cytokeratin 14 antibody [KRT14/532]

GTX34630_20170712_IHC-P_1_18121410_568.jpg
GTX34630_20170712_IHC-P_18121410_789.jpg

Cat No. GTX34630

Host Mouse
Clonality Monoclonal
Clone Name KRT14/532
Isotype IgG3
Application IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

IHC-P:0.5-1.0ug/ml for 30 min at RT.

Calculated MW

52 kDa. ( Note )

Specificity/Sensitivity

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human KRT14 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 14,Ck14,Ebs3,Ebs4,K14,Nfj,Krt14

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34630_20170712_IHC-P_1_18121410_568.jpg

GTX34630 IHC-P Image

Formalin-fixed, paraffin-embedded human Cervix stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).

GTX34630_20170712_IHC-P_18121410_789.jpg

GTX34630 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).

Package List Price ($)
$ 329