GeneTex

  • Country / Location Selection

United States (US)

Cytokeratin 14 antibody [LL002]

Anti-Cytokeratin 14 antibody [LL002] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34631
Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX34631
Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX34631
Anti-Cytokeratin 14 antibody [LL002] used in Flow cytometry (FACS). GTX34631

Cat. No. GTX34631

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LL002

Isotype

 IgG3

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($529)
See all Cytokeratin 14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK14 , EBS3 , EBS4 , K14 , KRT14 , NFJ , keratin 14 , Cytokeratin 14

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 14 antibody [LL002] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34631

GTX34631 ICC/IF Image

ICC/IF analysis of A549 cells using GTX34631 Cytokeratin 14 antibody [LL002].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX34631

GTX34631 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34631 Cytokeratin 14 antibody [LL002].

Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX34631

GTX34631 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34631 Cytokeratin 14 antibody [LL002].

Anti-Cytokeratin 14 antibody [LL002] used in Flow cytometry (FACS). GTX34631

GTX34631 FACS Image

FACS analysis of trypsinized MeOH-fixed HeLa cells using GTX34631 Cytokeratin 14 antibody [LL002].
Blue : Primary antibody
Red : Isotype control

REFERENCE

Clone Reference

Morita N et al. Med Mol Morphol 2020; (Epub) Keratinocytes from Gorlin Syndrome-induced pluripotent stem cells are resistant against UV radiation.
Submit a Reference

REVIEW

There are currently no reviews for Cytokeratin 14 antibody [LL002] (GTX34631). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529