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Cytokeratin 14 antibody [LL002]

Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX76603
Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX76603
Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX76603

Cat. No. GTX76603

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LL002

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Dog, Pig, Elephant, Lion
Package
40 μg ($169)
See all Cytokeratin 14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/200
IHC-Fr Assay dependent
FACS 1/100

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Skin

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.

Purification

Purified by ion exchange chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377444

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX76603

GTX76603 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX76603 Cytokeratin 14 antibody. Antigen retrieval using citrate buffer at pH6.2.

Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX76603

GTX76603 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX76603 Cytokeratin 14 antibody. Antigen retrieval using citrate buffer at pH6.0.

Anti-Cytokeratin 14 antibody [LL002] used in IHC (Paraffin sections) (IHC-P). GTX76603

GTX76603 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX76603 Cytokeratin 14 antibody. Antigen retrieval using citrate buffer at pH6.2.

REFERENCE

Application Reference

Morita N et al. Med Mol Morphol 2020; (Epub) Keratinocytes from Gorlin Syndrome-induced pluripotent stem cells are resistant against UV radiation.
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169