Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Cytokeratin 14(GTX112976) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX112976 diluted at 1:1000

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX112976) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.