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Cytokeratin 14 antibody

Anti-Cytokeratin 14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104124
Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124
Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

Cat No. GTX104124

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa ,* rat olfactory epithelium

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

keratin 14 , CK14 , EBS3 , EBS4 , K14 , NFJ

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Cytokeratin 14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104124

GTX104124 ICC/IF Image

Cytokeratin 14 antibody detects Cytokeratin 14 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody (GTX104124) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Cytokeratin 14 antibody used in Western Blot (WB). GTX104124

GTX104124 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (GTX104124) diluted at 1:6000.

Anti-Cytokeratin 14 antibody used in IHC (Paraffin sections) (IHC-P). GTX104124

GTX104124 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Lung CA, using Cytokeratin 14(GTX104124) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Cytokeratin 14 antibody Cat No. GTX104124

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 11-Dec-2013
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : olfactory epithelium tissue
Blocking : 5% FBS, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : citric acid, pH6
Primary Antibody : 1:200, 4°C, 20Hr
Figure Note : IHC analysis of OCT-embedded rat olfactory epithelium,using GTX104124(green).
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169