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Cytokeratin 15 antibody

Anti-Cytokeratin 15 antibody used in Western Blot (WB). GTX100572
Anti-Cytokeratin 15 antibody used in IHC (Paraffin sections) (IHC-P). GTX100572

Cat. No. GTX100572

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950641

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 15 , CK15 , K15 , K1CO

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 15 antibody used in Western Blot (WB). GTX100572

GTX100572 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX100572 diluted at 1:1000

Anti-Cytokeratin 15 antibody used in IHC (Paraffin sections) (IHC-P). GTX100572

GTX100572 IHC-P Image

Cytokeratin 15 antibody detects KRT15 protein at cytosol and membrane on HSC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HSC-3 xenograft.
Cytokeratin 15 antibody (GTX100572) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169