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Cytokeratin 17 antibody [SPM560]

Anti-Cytokeratin 17 antibody [SPM560] used in Western Blot (WB). GTX34635
Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635
Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635

Cat No. GTX34635

Host Mouse
Clonality Monoclonal
Clone Name SPM560
Isotype IgG2b
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Rat, Goat, Bovine, Pig
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
IHC-Fr 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5&6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20% of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Rat

Immunogen

The cytoskeletal fraction of rat colon epithelium

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 17 , 39.1 , Ck-17 , K17 , Pc , Pc2 , Pchc1 , Krt17

Background

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]

Database

DATA IMAGES
Anti-Cytokeratin 17 antibody [SPM560] used in Western Blot (WB). GTX34635

GTX34635 WB Image

Western Blot of HeLa Lysate using CK17 Monoclonal Antibody (SPM560).

Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635

GTX34635 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with CK17 Monoclonal Antibody (SPM560).

Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635

GTX34635 IHC-P Image

Formalin-fixed, paraffin-embedded human Skin stained with CK17 Monoclonal Antibody (SPM560).

REFERENCE
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REVIEW
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Package List Price ($)
$ 429