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Cytokeratin 17 antibody [SPM560]

Anti-Cytokeratin 17 antibody [SPM560] used in Flow cytometry (FACS). GTX34635
Anti-Cytokeratin 17 antibody [SPM560] used in Western Blot (WB). GTX34635
Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635
Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635

Cat No. GTX34635

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM560

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Rat, Goat, Bovine, Pig
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

The cytoskeletal fraction of rat colon epithelium

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 17 antibody [SPM560] used in Flow cytometry (FACS). GTX34635

GTX34635 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX34635 Cytokeratin 17 antibody [SPM560].
Blue : Primary antibody
Red : Isotype control

Anti-Cytokeratin 17 antibody [SPM560] used in Western Blot (WB). GTX34635

GTX34635 WB Image

WB analysis of HeLa cell lysate using GTX34635 Cytokeratin 17 antibody [SPM560].

Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635

GTX34635 IHC-P Image

IHC-P analysis of human skin tissue using GTX34635 Cytokeratin 17 antibody [SPM560].

Anti-Cytokeratin 17 antibody [SPM560] used in IHC (Paraffin sections) (IHC-P). GTX34635

GTX34635 IHC-P Image

IHC-P analysis of human skin tissue using GTX34635 Cytokeratin 17 antibody [SPM560].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339