GeneTex
  • Country / Location Selection

United States (US)

Cytokeratin 17 antibody

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765
Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765
Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765
Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765
Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765
Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765
Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765

Cat. No. GTX103765

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Rat bladder

Predict Reactivity

Sheep, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950643

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 17 , 39.1 , CK-17 , K17 , PC , PC2 , PCHC1

Cellular Localization

Cytoplasm

Background

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

GTX103765 IHC-P Image

Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Cytokeratin 17 antibody (GTX103765) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765

GTX103765 WB Image

Sample (50 ug of whole cell lysate)
A: Rat bladder
10% SDS PAGE
GTX103765 diluted at 1:10000

Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765

GTX103765 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cytokeratin 17(GTX103765) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

GTX103765 IHC-P Image

Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Cytokeratin 17 antibody (GTX103765) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

GTX103765 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using Cytokeratin 17(GTX103765) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765

GTX103765 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX103765 diluted at 1:5000

Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765

GTX103765 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 28652576

REFERENCE

REVIEW

Cytokeratin 17 antibody Cat. No. GTX103765

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCaT cell
Amount used : 20ug
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169