Cytokeratin 17 antibody

Name: Cytokeratin 17 antibody
Brand: GeneTex
SKU: GTX103765
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765

Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765

Cat. No. GTX103765

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Cytokeratin 17 products

View product citations for antibody GTX103765 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Rat bladder

Predict Reactivity

Sheep, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950643

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 17 , 39.1 , CK-17 , K17 , PC , PC2 , PCHC1

Cellular Localization

Cytoplasm

Background

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq]

Database

Gene ID: 3872 KRT17
UniProt: Q04695 KRT17

Research Area

DATA IMAGES

GTX103765 IHC-P Image

Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Cytokeratin 17 antibody (GTX103765) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103765 WB Image

Sample (50 ug of whole cell lysate)
A: Rat bladder
10% SDS PAGE
GTX103765 diluted at 1:10000

GTX103765 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cytokeratin 17(GTX103765) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX103765 IHC-P Image

Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Cytokeratin 17 antibody (GTX103765) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103765 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using Cytokeratin 17(GTX103765) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103765 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX103765 diluted at 1:5000

GTX103765 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 28652576

REFERENCE

Application Reference

Gopinathan P et al. Adv Healthc Mater 2020; 9 (10) : e1901875 Exploring Circulating Tumor Cells in Cholangiocarcinoma Using a Novel Glycosaminoglycan Probe on a Microfluidic Platform.

Application : ICC/IF

Reactivity : Human

Hung LY et al. Sci Rep 2017; 7 (1) : 4248 A Microfluidic Chip for Detecting Cholangiocarcinoma Cells in Human Bile.

Application : ICC/IF

Reactivity : Human

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