GeneTex
  • Country / Location Selection

United States (US)

Cytokeratin 17 antibody

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765
Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765
Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765
Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765
Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765
Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

Cat No. GTX103765

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , Rat bladder

Predict Reactivity

Sheep, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

keratin 17 , 39.1 , CK-17 , K17 , PC , PC2 , PCHC1

Cellular Localization

Cytoplasm

Background

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq]

Database

DATA IMAGES
Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

GTX103765 IHC-P Image

Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Cytokeratin 17 antibody (GTX103765) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 17 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103765

GTX103765 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cytokeratin 17(GTX103765) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

GTX103765 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using Cytokeratin 17(GTX103765) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765

GTX103765 WB Image

Sample (50 ug of whole cell lysate)
A: Rat bladder
10% SDS PAGE
GTX103765 diluted at 1:10000

Anti-Cytokeratin 17 antibody used in Western Blot (WB). GTX103765

GTX103765 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX103765 diluted at 1:10000

Anti-Cytokeratin 17 antibody used in IHC (Paraffin sections) (IHC-P). GTX103765

GTX103765 IHC-P Image

Cytokeratin 17 antibody detects Cytokeratin 17 protein at cytoplasm in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Cytokeratin 17 antibody (GTX103765) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
There are currently no reviews for Cytokeratin 17 antibody (GTX103765). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169