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Cytokeratin 18 antibody [1E1]

Anti-Cytokeratin 18 antibody [1E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256
Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

Cat No. GTX84256

Host

Mouse

Clonality

Monoclonal

Clone Name

1E1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Monkey
Package
100 μl ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10726718

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Nucleolus

Background

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 antibody [1E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84256

GTX84256 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Cytokeratin 18 plasmid using GTX84256 Cytokeratin 18 antibody [1E1].

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of HEK293T cells transfected with Cytokeratin 18 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 5 ug per lane

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of various cell lines using GTX84256 Cytokeratin 18 antibody [1E1].
Loading : 35 ug per lane

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human breast tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in Western Blot (WB). GTX84256

GTX84256 WB Image

WB analysis of HepG2 cell lysate using GTX84256 Cytokeratin 18 antibody [1E1].
Dilution : 1:1000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120ººC for 3 min.
Dilution : 1:6000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human colon tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120ººC for 3 min.
Dilution : 1:6000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human liver tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120ººC for 3 min.
Dilution : 1:6000

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Cytokeratin 18 antibody [1E1] used in IHC (Paraffin sections) (IHC-P). GTX84256

GTX84256 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84256 Cytokeratin 18 antibody [1E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

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SDS
PBS.pdf
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Sodium Azide.pdf