Cytokeratin 18 antibody [Cocktail]

Cat No. GTX34645

Host	Mouse
Clonality	Monoclonal
Clone Name	Cocktail
Isotype	IgG1
Application	WB, IHC-P, FACS
Reactivity	Human
Package	100 μg ($339)

See all Cytokeratin 18 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 min at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human KRT18 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18

Cellular Localization

Cytoplasm,Perinuclear region,Nucleus,Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3875 KRT18
UniProt: P05783 KRT18

Research Area

DATA IMAGES

GTX34645 WB Image

WB analysis of HCT116 cell lysate using GTX34645 Cytokeratin 18 antibody [Cocktail].

GTX34645 IHC-P Image

IHC-P analysis of human cervical ca tissue using GTX34645 Cytokeratin 18 antibody [Cocktail].

GTX34645 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34645 Cytokeratin 18 antibody [Cocktail].

GTX34645 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX34645 Cytokeratin 18 antibody [Cocktail].

GTX34645 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34645 Cytokeratin 18 antibody [Cocktail].

GTX34645 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX34645 Cytokeratin 18 antibody [Cocktail].

GTX34645 FACS Image

FACS analysis of PFA-fixed HeLa cells using GTX34645 Cytokeratin 18 antibody [Cocktail].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 339

Qty

Cytokeratin 18 antibody [Cocktail]

Cat No. GTX34645

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Specificity/Sensitivity

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX34645 WB Image

GTX34645 IHC-P Image

GTX34645 IHC-P Image

GTX34645 IHC-P Image

GTX34645 IHC-P Image

GTX34645 IHC-P Image

GTX34645 FACS Image

REFERENCE

REVIEW

Pamphlet - Recombinant Active Proteins

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