*Optimal dilutions/concentrations should be determined by the researcher.
|2-4μg/ml for 30 min at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
We do not recommend use of this product for Rat.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
GTX34641 WB Image
WB analysis of HCT116 cell lysate using GTX34641 Cytokeratin 18 antibody [DE-K18].
GTX34641 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34641 Cytokeratin 18 antibody [DE-K18].
Green : Primary antibody
Red : nucleus
GTX34641 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34641 Cytokeratin 18 antibody [DE-K18].
GTX34641 WB Image
WB analysis of MCF-7 cell lysate using GTX34641 Cytokeratin 18 antibody [DE-K18].
GTX34641 FACS Image
FACS analysis of trypsinised MeOH-fixed HeLa cells using GTX34641 Cytokeratin 18 antibody [DE-K18].
Blue : Primary antibody
Red : Isotype control