APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 2-4μg/ml for 30 min at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
We do not recommend use of this product for Rat samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18
Cellular Localization
Cytoplasm,Perinuclear region,Nucleus,Nucleolus
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 18 antibody [DE-K18] used in Flow cytometry (FACS). GTX34641](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 18 antibody [DE-K18] used in Flow cytometry (FACS). GTX34641")
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GTX34641 FACS Image
FACS analysis of trypsinised MeOH-fixed HeLa cells using GTX34641 Cytokeratin 18 antibody [DE-K18].
Blue : Primary antibody
Red : Isotype control
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GTX34641 WB Image
WB analysis of HCT116 cell lysate using GTX34641 Cytokeratin 18 antibody [DE-K18].
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GTX34641 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34641 Cytokeratin 18 antibody [DE-K18].
Green : Primary antibody
Red : nucleus
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GTX34641 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34641 Cytokeratin 18 antibody [DE-K18].
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GTX34641 WB Image
WB analysis of MCF-7 cell lysate using GTX34641 Cytokeratin 18 antibody [DE-K18].
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REFERENCE
There are currently no references for Cytokeratin 18 antibody [DE-K18] (GTX34641). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 18 antibody [DE-K18] (GTX34641). Be the first to share your experience with this product.
![Anti-Cytokeratin 18 antibody [DE-K18] used in Flow cytometry (FACS). GTX34641](/upload/website/prouct_img/small/GTX34641/GTX34641_20200115_FACS_1895_w_23051500_193.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Flow cytometry (FACS). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641](/upload/website/prouct_img/small/GTX34641/GTX34641_20200115_WB_85_w_23051500_733.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34641](/upload/website/prouct_img/small/GTX34641/GTX34641_20200115_ICC-IF_225_w_23051500_647.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in IHC (Paraffin sections) (IHC-P). GTX34641](/upload/website/prouct_img/small/GTX34641/GTX34641_20200115_IHC-P_453_w_23051500_445.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in IHC (Paraffin sections) (IHC-P). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641](/upload/website/prouct_img/small/GTX34641/GTX34641_20200115_WB_1547_w_23051500_300.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Flow cytometry (FACS). GTX34641](/upload/website/prouct_img/normal/GTX34641/GTX34641_20200115_FACS_1895_w_23051500_193.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Flow cytometry (FACS). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641](/upload/website/prouct_img/normal/GTX34641/GTX34641_20200115_WB_85_w_23051500_733.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34641](/upload/website/prouct_img/normal/GTX34641/GTX34641_20200115_ICC-IF_225_w_23051500_647.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in IHC (Paraffin sections) (IHC-P). GTX34641](/upload/website/prouct_img/normal/GTX34641/GTX34641_20200115_IHC-P_453_w_23051500_445.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in IHC (Paraffin sections) (IHC-P). GTX34641")
![Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641](/upload/website/prouct_img/normal/GTX34641/GTX34641_20200115_WB_1547_w_23051500_300.webp "Anti-Cytokeratin 18 antibody [DE-K18] used in Western Blot (WB). GTX34641")