GeneTex
  • Country / Location Selection

United States (US)

Cytokeratin 18 antibody [KRT18/1190]

Anti-Cytokeratin 18 antibody [KRT18/1190] used in Western Blot (WB). GTX34637
Anti-Cytokeratin 18 antibody [KRT18/1190] used in IHC (Paraffin sections) (IHC-P). GTX34637
Anti-Cytokeratin 18 antibody [KRT18/1190] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34637
Anti-Cytokeratin 18 antibody [KRT18/1190] used in Western Blot (WB). GTX34637
Anti-Cytokeratin 18 antibody [KRT18/1190] used in Western Blot (WB). GTX34637
Anti-Cytokeratin 18 antibody [KRT18/1190] used in IHC (Paraffin sections) (IHC-P). GTX34637

Cat No. GTX34637

Host

Mouse

Clonality

Monoclonal

Clone Name

KRT18/1190

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human KRT18 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18

Cellular Localization

Cytoplasm,Perinuclear region,Nucleus,Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 antibody [KRT18/1190] used in Western Blot (WB). GTX34637

GTX34637 WB Image

WB analysis of HCT116 cell lysate using GTX34637 Cytokeratin 18 antibody [KRT18/1190].

Anti-Cytokeratin 18 antibody [KRT18/1190] used in IHC (Paraffin sections) (IHC-P). GTX34637

GTX34637 IHC-P Image

IHC-P analysis of rat colon tissue using GTX34637 Cytokeratin 18 antibody [KRT18/1190].

Anti-Cytokeratin 18 antibody [KRT18/1190] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34637

GTX34637 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34637 Cytokeratin 18 antibody [KRT18/1190].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 18 antibody [KRT18/1190] used in Western Blot (WB). GTX34637

GTX34637 WB Image

WB analysis of human (A) stomach (B) intestine lysates using GTX34637 Cytokeratin 18 antibody [KRT18/1190].

Anti-Cytokeratin 18 antibody [KRT18/1190] used in Western Blot (WB). GTX34637

GTX34637 WB Image

WB analysis of human A549 and HepG2 cell lysates using GTX34637 Cytokeratin 18 antibody [KRT18/1190].

Anti-Cytokeratin 18 antibody [KRT18/1190] used in IHC (Paraffin sections) (IHC-P). GTX34637

GTX34637 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34637 Cytokeratin 18 antibody [KRT18/1190].

REFERENCE

There are currently no references for Cytokeratin 18 antibody [KRT18/1190] (GTX34637). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cytokeratin 18 antibody [KRT18/1190] (GTX34637). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339