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Cytokeratin 18 antibody [KRT18/2808R]

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657
Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657
Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657
Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657
Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Western Blot (WB). GTX02657
Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02657

Cat No. GTX02657

Host

Rabbit

Clonality

Monoclonal

Clone Name

KRT18/2808R

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length KRT18 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657

GTX02657 FACS Image

FACS analysis of HeLa cells using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
Blue : Primary antibody
Red : Isotype control

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657

GTX02657 IHC-P Image

IHC-P analysis of human thyroid carcinoma section using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657

GTX02657 IHC-P Image

IHC-P analysis of human colon carcinoma section using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657

GTX02657 IHC-P Image

IHC-P analysis of human colon carcinoma section using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Western Blot (WB). GTX02657

GTX02657 WB Image

WB analysis of HCT116 whole cell lysate using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].

Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02657

GTX02657 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
Green : Primary antibody
Red : nuclear counterstain

REFERENCE

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REVIEW

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