APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2 μg/ml |
| 1-2 μg/ml |
| 1-2 μg/ml |
| 1-2 μg/million cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length KRT18 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
keratin 18 , CK-18 , CYK18 , K18
Cellular Localization
Nucleolus
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657")
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GTX02657 FACS Image
FACS analysis of HeLa cells using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
Blue : Primary antibody
Red : Isotype control
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GTX02657 IHC-P Image
IHC-P analysis of human colon carcinoma section using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
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GTX02657 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
Green : Primary antibody
Red : nuclear counterstain
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GTX02657 IHC-P Image
IHC-P analysis of human thyroid carcinoma section using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
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GTX02657 WB Image
WB analysis of HCT116 whole cell lysate using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
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GTX02657 IHC-P Image
IHC-P analysis of human colon carcinoma section using GTX02657 Cytokeratin 18 antibody [KRT18/2808R].
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REFERENCE
There are currently no references for Cytokeratin 18 antibody [KRT18/2808R] (GTX02657). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 18 antibody [KRT18/2808R] (GTX02657). Be the first to share your experience with this product.
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657](/upload/website/prouct_img/small/GTX02657/GTX02657_20210319_FACS_w_23053122_467.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657](/upload/website/prouct_img/small/GTX02657/GTX02657_20210319_IHC-P_2_w_23053122_360.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02657](/upload/website/prouct_img/small/GTX02657/GTX02657_20210319_ICCIF_w_23053122_367.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657](/upload/website/prouct_img/small/GTX02657/GTX02657_20210319_IHC-P_w_23053122_778.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Western Blot (WB). GTX02657](/upload/website/prouct_img/small/GTX02657/GTX02657_20210319_WB_w_23053122_597.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Western Blot (WB). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657](/upload/website/prouct_img/small/GTX02657/GTX02657_20210319_IHC-P_1_w_23053122_325.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657](/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_FACS_w_23053122_467.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Flow cytometry (FACS). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657](/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_IHC-P_2_w_23053122_360.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02657](/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_ICCIF_w_23053122_367.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657](/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_IHC-P_w_23053122_778.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Western Blot (WB). GTX02657](/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_WB_w_23053122_597.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in Western Blot (WB). GTX02657")
![Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657](/upload/website/prouct_img/normal/GTX02657/GTX02657_20210319_IHC-P_1_w_23053122_325.webp "Anti-Cytokeratin 18 antibody [KRT18/2808R] used in IHC (Paraffin sections) (IHC-P). GTX02657")