Cytokeratin 18 antibody [KRT18/2819R]

Cat. No. GTX02658

Host	Rabbit
Clonality	Monoclonal
Clone Name	KRT18/2819R
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Goat, Sheep, Bovine, Cat, Dog, Hamster, Pig, Ferret, Horse, Marmoset
Package	100 μg ($529)

See all Cytokeratin 18 products

Datasheet

View product citations for antibody GTX02658 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	1-2 μg/ml
IHC-P	1-2 μg/ml
FACS	1-2 μg/million cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
We do not recommend use of this product for Chicken,Xenopus samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length KRT18 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3875 KRT18
UniProt: P05783 KRT18

Research Area

DATA IMAGES

GTX02658 IHC-P Image

IHC-P analysis of human colon carcinoma section using GTX02658 Cytokeratin 18 antibody [KRT18/2819R].

GTX02658 WB Image

WB analysis of HCT116 whole cell lysate using GTX02658 Cytokeratin 18 antibody [KRT18/2819R].

GTX02658 FACS Image

FACS analysis of PFA-fixed HeLa cells using GTX02658 Cytokeratin 18 antibody [KRT18/2819R].
Blue : Primary antibody
Red : Isotype control

GTX02658 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX02658 Cytokeratin 18 antibody [KRT18/2819R].
Blue : Primary antibody
Red : nuclear counterstain

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty