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Cytokeratin 18 antibody [KRT18/835]

Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in Protein Array (Protein Array). GTX34643
Anti-Cytokeratin 18 antibody [KRT18/835] used in Western Blot (WB). GTX34643

Cat No. GTX34643

Host

Mouse

Clonality

Monoclonal

Clone Name

KRT18/835

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, Protein Array

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF Assay dependent
IHC-P 1-2μg/ml for 30 min at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
This product does not react with Rat,Sheep,Bovine,Dog,Hamster,Pig.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant full-length human KRT18 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18

Cellular Localization

Cytoplasm,Perinuclear region,Nucleus,Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643

GTX34643 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].

Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643

GTX34643 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].

Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643

GTX34643 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].

Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643

GTX34643 IHC-P Image

IHC-P analysis of human prostate cancer tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].

Anti-Cytokeratin 18 antibody [KRT18/835] used in IHC (Paraffin sections) (IHC-P). GTX34643

GTX34643 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX34643 Cytokeratin 18 antibody [KRT18/835].

Anti-Cytokeratin 18 antibody [KRT18/835] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34643

GTX34643 ICC/IF Image

ICC/IF analysis of MCF cells using GTX34643 Cytokeratin 18 antibody [KRT18/835].
Green : Primary antibody
Red : nucleus

Anti-Cytokeratin 18 antibody [KRT18/835] used in Protein Array (Protein Array). GTX34643

GTX34643 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/835). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Cytokeratin 18 antibody [KRT18/835] used in Western Blot (WB). GTX34643

GTX34643 WB Image

WB analysis of HCT116 cell lysate using GTX34643 Cytokeratin 18 antibody [KRT18/835].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339