Cytokeratin 18 antibody [KRT18/836]

Cat. No. GTX34644

Host	Mouse
Clonality	Monoclonal
Clone Name	KRT18/836
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, Protein Array
Reactivity	Human, Cat, Dog
Package	100 μg ($529)

See all Cytokeratin 18 products

Datasheet
Document

View product citations for antibody GTX34644 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 min at RT
FACS	1-2μg/10⁶ cells
Protein Array	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.
We do not recommend use of this product for Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human KRT18 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CK18 , CYK18 , K18 , KRT18 , keratin 18 , Cytokeratin 18

Cellular Localization

Cytoplasm,Perinuclear region,Nucleus,Nucleolus

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3875 KRT18
UniProt: P05783 KRT18

Research Area

DATA IMAGES

GTX34644 WB Image

WB analysis of HCT116 cell lysate using GTX34644 Cytokeratin 18 antibody [KRT18/836].

GTX34644 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34644 Cytokeratin 18 antibody [KRT18/836].

GTX34644 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/836). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

GTX34644 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34644 Cytokeratin 18 antibody [KRT18/836].
Green : Primary antibody
Red : nucleus

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty