PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. Please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Liver: At least a weak to moderate, predominantly membranous staining should be seen in virtually all hepatocytes. Appendix: A strong staining should be seen in all epithelial cells.
Negative Control
Appendix: Staining should be absent in all non-epithelial cells of the appendix.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length KRT18 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
keratin 18 , CK-18 , CYK18 , K18
Cellular Localization
Cytoplasm, perinuclear region , Nucleolus
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/templates/default/js/lightbox/images/loading.gif "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")
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GTX04374 IHC-P Image
IHC-P analysis of human epididymis tissue using GTX04374 Cytokeratin 18 antibody [MSVA-118R] HistoMAX™.
All epithelial cells are cytokeratin 18 positive.
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GTX04374 IHC-P Image
IHC-P analysis of human endocervix tissue using GTX04374 Cytokeratin 18 antibody [MSVA-118R] HistoMAX™.
Strong cytokeratin 18 staining of all epithelial cells.
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GTX04374 IHC-P Image
IHC-P analysis of human gastric antrum tissue using GTX04374 Cytokeratin 18 antibody [MSVA-118R] HistoMAX™.
Strong cytokeratin 18 staining of all epithelial cells.
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REFERENCE
There are currently no references for Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ (GTX04374). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ (GTX04374). Be the first to share your experience with this product.
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/upload/website/prouct_img/small/GTX04374/GTX04374_20230728_IHC-P_169_23072722_715.webp "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/upload/website/prouct_img/small/GTX04374/GTX04374_20230728_IHC-P_299_23072723_428.webp "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/upload/website/prouct_img/small/GTX04374/GTX04374_20230728_IHC-P_39_23072722_592.webp "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/upload/website/prouct_img/normal/GTX04374/GTX04374_20230728_IHC-P_169_23072722_715.webp "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/upload/website/prouct_img/normal/GTX04374/GTX04374_20230728_IHC-P_299_23072723_428.webp "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")
![Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374](/upload/website/prouct_img/normal/GTX04374/GTX04374_20230728_IHC-P_39_23072722_592.webp "Anti-Cytokeratin 18 antibody [MSVA-118R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04374")