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Cytokeratin 18 antibody [N3C3]

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX112978
Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

Cat No. GTX112978

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

Human, Mouse, Zebrafish, Dog
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Raji , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950644

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Cytoplasm , perinuclear region

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

GTX112978 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody [N3C3] detects Cytokeratin 18 protein at cytoplasm in mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Cytokeratin 18(GTX112978) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978

GTX112978 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX112978) antibody at 1:200 dilution.

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

GTX112978 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX112978 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978

GTX112978 ICC/IF Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant nephroblastoma.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

GTX112978 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX112978

GTX112978 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Cytokeratin 18 antibody [N3C3] (GTX112978) at 1:200 dilution.

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog stomach.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169