Cytokeratin 18 antibody [N3C3]

Name: Cytokeratin 18 antibody [N3C3]
Brand: GeneTex
SKU: GTX112978
Price: 399.00 USD

Anti-Cytokeratin 18 antibody [N3C3] used in Western Blot (WB). GTX112978

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112978

Anti-Cytokeratin 18 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112978

Anti-Cytokeratin 18 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX112978

Cat. No. GTX112978

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm
Reactivity	Human, Mouse, Zebrafish, Dog
Package	100 μl ($399), 25 μl ($169)

See all Cytokeratin 18 products

View product citations for antibody GTX112978 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Raji , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950644

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Cytoplasm , perinuclear region

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3875 KRT18
UniProt: P05783 KRT18

Research Area

DATA IMAGES

GTX112978 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112978 ICC/IF Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant nephroblastoma.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112978 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX112978) diluted at 1:8000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112978 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Cytokeratin 18 antibody [N3C3] (GTX112978) at 1:200 dilution.

GTX112978 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog stomach.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX112978) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112978 IHC-P Image

Cytokeratin 18 antibody [N3C3] detects Cytokeratin 18 protein at cytoplasm in mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Cytokeratin 18 antibody [N3C3] (GTX112978) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112978 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX112978) antibody at 1:200 dilution.

GTX112978 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
10% SDS PAGE
GTX112978 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112978 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Cytokeratin 18(GTX112978) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Nguyen QH et al. Nat Commun 2018; 9 (1) : 2028 Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Application : IHC-P

Reactivity : Human

Submit a Reference

REVIEW

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Application Reference

Nguyen QH et al. Nat Commun 2018; 9 (1):2028 Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Application : IHC-P

Reactivity : Human

SDS
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Cytokeratin 18 antibody [N3C3]

Cat. No. GTX112978

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX112978 WB Image

GTX112978 IHC-P Image

GTX112978 ICC/IF Image

GTX112978 IHC-P Image

GTX112978 WB Image

GTX112978 IHC-P Image

GTX112978 IHC-P Image

GTX112978 IHC-Wm Image

GTX112978 IHC-P Image

GTX112978 IHC-P Image

GTX112978 ICC/IF Image

GTX112978 WB Image

GTX112978 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.